Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells

Zacharias Da

(2002). Science, 296(5569) , 913-916. doi: 10.1126/science.1068539. Article   Pubmed

    Primary Proteins:
  1. mCitrine
  2. mEGFP
  3. mEYFP
    Secondary Proteins:
  1. EYFP
Add photostability measurements


We replaced hydrophobic residues at the crystallographic interface of the dimer with positively charged residues (A206K, L221K, or F223R). These mutants were likewise expressed and purified, and their self-association was measured by analytical ultracentrifugation... We call these novel monomeric GFP variants mCFP, mGFP, and mYFP. These mutations caused no significant alterations in the spectral properties.