Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Entacmaea quadricolor | 26.4 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
403 | 454 | 80,900 | 0.76 | 61.48 | 3.4 |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
610.0 | 4.25 (mW/mm2) | LED | Widefield | HEK293T | 21.0 | Papadaki et al. (2022) | |
1466.0 | 0.91 (mW/mm2) | LED | Widefield | HEK293T | 21.0 | Papadaki et al. (2022) | |
200.0 | 3.72 (mW/mm2) | LED | Widefield | HEK293T | 21.0 | Papadaki et al. (2022) |
Electra2 was derived from mRuby3 with the following mutations: K6E/M63L/Y64F/R67K/K81E/E114G/P127H/N129K/K138E/N143F/C172A/F174I/H197Y/Q213L/Y231aF
amino acid numbers relative to eqFP611. show relative to mRuby3
When transiently expressed in HEK cells, Electras were more than fourfold brighter than mBlueberry2 and about 1.4-fold brighter than EBFP2, while slightly dimmer than mTagBFP2 (Fig. 3a, Table 1).
Papadaki et al. (2022)
(2022). Scientific Reports, 12(1) , 10190. doi: 10.1038/s41598-022-13214-0. Article Pubmed
Something missing or incorrect? Submit a change Submit a change