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Comparison List

SCFP3A

SCFP3A is a basic (constitutively fluorescent) cyan fluorescent protein published in 2006, derived from Aequorea victoria. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

FPbase ID: HFE84

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
433 474 30,000 0.56 16.8 4.5 6.4 2.6

Photostability

No photostability measurements available ... add one!

SCFP3A Sequence

SCFP3A was derived from SCFP2 with the following mutations: H148D
amino acid numbers relative to avGFP. show relative to SCFP2

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
GenBank: AAZ65848
IPG: 4596895

Structure

Deposited: ,
Chromophore (TWG):

Excerpts

We created monomeric optimized variants of ECFP and EYFP, which fold faster and more efficiently at 37°C and have superior solubility and brightness. Bacteria expressing SCFP3A were 9-fold brighter than those expressing ECFP and 1.2-fold brighter than bacteria expressing Cerulean. In HeLa cells, the improvements were less pronounced; nonetheless, cells expressing SCFP3A and SYFP2 were both 1.5-fold brighter than cells expressing ECFP and EYFP(Q69K), respectively.

Kremers et al. (2006)

Primary Reference

Additional References

External Resources

Change history

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