High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

Zhong H, Ceballos Cc, Massengill Ci, Muniak Ma, Ma L, Qin M, Petrie Sk, Mao T

(2021). eLife, 10, . doi: 10.7554/elife.64911. Article

    Secondary Proteins:
  1. mNeonGreen,
  2. mTurquoise2
Add photostability measurements

Excerpts

Notably, when both mTurquoise2 and mNeonGreen donors were included simultaneously, double-labeled cells could be found, which appeared to be capable of dividing (Figure 4—figure supplement 1), indicating that FP labeling does not affect cell viability in diploid CRISPIEd cells

Notably, when both mTurquoise2 and mNeonGreen donors were included simultaneously, double-labeled cells could be found, which appeared to be capable of dividing (Figure 4—figure supplement 1), indicating that FP labeling does not affect cell viability in diploid CRISPIEd cells