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Electra2

Electra2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2022, derived from Entacmaea quadricolor.
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Electra2 Spectrum Fluorescent protein Electra2 excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.4 kDa -

FPbase ID: 5GJX3

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
403 454 80,900 0.76 61.48     3.4
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
610.0 4.25 (mW/mm2) LED Widefield HEK293T 21.0 Papadaki et al. (2022)
1466.0 0.91 (mW/mm2) LED Widefield HEK293T 21.0 Papadaki et al. (2022)
200.0 3.72 (mW/mm2) LED Widefield HEK293T 21.0 Papadaki et al. (2022)
A caution on interpretation of photostability measurements
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Electra2 Sequence

BLAST

Electra2 was derived from mRuby3 with the following mutations: K6E/M63L/Y64F/R67K/K81E/E114G/P127H/N129K/K138E/N143F/C172A/F174I/H197Y/Q213L/Y231aF
amino acid numbers relative to eqFP611. show relative to mRuby3

MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTDIALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAVAKYSNLGGGMDELFK
GenBank: OL631607

Excerpts

When transiently expressed in HEK cells, Electras were more than fourfold brighter than mBlueberry2 and about 1.4-fold brighter than EBFP2, while slightly dimmer than mTagBFP2 (Fig. 3a, Table 1).

Papadaki et al. (2022)

Primary Reference

Dual-expression system for blue fluorescent protein optimization

Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia J-M, Köster Rw, Namikawa K, Piatkevich Kd

(2022). Scientific Reports, 12(1) , 10190. doi: 10.1038/s41598-022-13214-0. Article   Pubmed

Additional References

    No additional references have been added.

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