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Comparison List

Electra1

Electra1 is a basic (constitutively fluorescent) blue fluorescent protein published in 2022, derived from Entacmaea quadricolor.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.4 kDa -

FPbase ID: 5X9A8

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
402 456          
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Photostability

No photostability measurements available ... add one!

Electra1 Sequence

BLAST

Electra1 was derived from mRuby3 with the following mutations: K6E/M63L/Y64F/R67K/E114G/K138E/N143F/D151N/G167D/C172A/F174I/N186D/H197Y/I202V/Q213L
amino acid numbers relative to eqFP611. show relative to mRuby3

MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGGLVYNVKVRGVNFPSNGPVMQKKTEGWEPFTEMMYPANGGLRGYTDIALKVDGDGHLHANIVTTYRSKKTVGDIKMPGVHAVDYRLERVEESDNETYVVLREVAVAKYSNLGGGMDELYK
GenBank: OL631606

Excerpts

Quantification of intracellular brightness revealed that Electra1 and Electra2 were 2.3- and 2.1-fold brighter than mTagBFP2

Papadaki et al. (2022)

Primary Reference

Dual-expression system for blue fluorescent protein optimization

Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, Jia J-M, Köster Rw, Namikawa K, Piatkevich Kd

(2022). Scientific Reports, 12(1) , 10190. doi: 10.1038/s41598-022-13214-0. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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