compare

Comparison List

Comparing:

mScarlet, mScarlet-I, mScarlet-H

You can share this comparison at: www.fpbase.org/compare/mscarlet,mscarlet-i,mscarlet-h

Attribute Comparison

Name λex λem StokesλΔ EC QY Brightness pKa Aggregation Maturation Lifetimeτ kDa
mScarlet 569 594 25 100,000 0.7 70.0 5.3 m 174.0 3.9 26.35
mScarlet-I 569 593 24 104,000 0.54 56.16 5.4 m 36.0 3.1 26.36
mScarlet-H 551 592 41 74,000 0.2 14.8 4.8 m 264.0 1.3 26.36

Sequence Comparison

mScarlet        MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWD
mScarlet-I      MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWD
mScarlet-H      MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWD

mScarlet        ILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLI
mScarlet-I      ILSPQFMYGSRAFiKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLI
mScarlet-H      ILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLI

mScarlet        YKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKT
mScarlet-I      YKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKT
mScarlet-H      YKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKhALRLKDGGRYLADFKT

mScarlet        TYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK
mScarlet-I      TYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK
mScarlet-H      TYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK

References

  1. Bindels et al. (2016) mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods.Journal   Pubmed

  2. Mastop et al. (2017) Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2. Scientific Reports.Journal   Pubmed

  3. Chertkova et al. (2017) Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells. .Journal

  4. Balleza et al. (2017) Systematic characterization of maturation time of fluorescent proteins in living cells. Nature Methods.Journal   Pubmed

  5. Dunsing et al. (2018) Optimal fluorescent protein tags for quantifying protein oligomerization in living cells. Scientific Reports.Journal   Pubmed

  6. Wang et al. (2018) Spying on protein interactions in living cells with reconstituted scarlet light. The Analyst.Journal