Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

Ai H-, Olenych Sg, Wong P, Davidson Mw, Campbell Re

(2008). BMC Biology, 6(1) , 13. doi: 10.1186/1741-7007-6-13. Article   Pubmed

    Primary Proteins:
  1. G1
  2. G2
  3. G3
  4. mTFP1-Y67H
  5. mTFP1-Y67W
  6. mWasabi

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mWasabi 93.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

The purified mTFP1-Y67H variant exhibited no significant fluorescence, but did have a strong absorbance peak that was blue-shifted by 10.5 nm (mean wavelength at half maximum intensity relative to avGFP-derived EBFP