A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications

Chang H, Zhang M, Ji W, Chen J, Zhang Y, Liu B, Lu J, Zhang J, Xu P, Xu T

(2012). Proceedings of the National Academy of Sciences, 109(12) , 4455-4460. doi: 10.1073/pnas.1113770109. Article   Pubmed

    Primary Proteins:
  1. mGeos-C
  2. mGeos-E
  3. mGeos-F
  4. mGeos-L
  5. mGeos-M
  6. mGeos-S
    Secondary Proteins:
  1. PDM1-4
Add photostability measurements


An attempt to find Dronpa variants with slower off-switching rate has led to the identification of PDM1-4. Unfortunately, PDM1-4 was prone to form tetramers and did not show improvement in photon output. In addition, PDM1-4 has worsened photostability and less contrast between on and off-states.