Superresolution Imaging of Multiple Fluorescent Proteins with Highly Overlapping Emission Spectra in Living Cells

Gunewardene Ms, Subach Fv, Gould Tj, Penoncello Gp, Gudheti Mv, Verkhusha Vv, Hess St

(2011). Biophysical Journal, 101(6) , 1522-1528. doi: 10.1016/j.bpj.2011.07.049. Article   Pubmed

    Primary Proteins:
  1. PAmKate
Add photostability measurements

Excerpts

To convert mKate into a PAFP, we mutated amino acid residues at positions 69, 84, 148, 165, 167, 181, and 203 (numbering follows the alignment with green fluorescent protein (GFP) , which were crucial for photoactivation of the chromophore in PAmCherry1.