PAmKate is a photoactivatable red fluorescent protein published in 2011, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
No photostability measurements available ...
PAmKate was derived from
mKate with the following mutations: F80W/S143N/S158G/*230LextN
amino acid numbers relative to
eqFP578. show relative to mKate
mKate into a PAFP, we mutated amino acid residues at positions 69, 84, 148, 165, 167, 181, and 203 (numbering follows the alignment with green fluorescent protein ( GFP) , which were crucial for photoactivation of the chromophore in PAmCherry1.
Gunewardene et al. (2011)
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Biophysical Journal, 101(6) , 1522-1528. doi: 10.1016/j.bpj.2011.07.049.