Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Entacmaea quadricolor | 26.3 kDa | - |
State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|---|
On | 586 | 628 | 25,000 | 0.18 | 4.5 | 5.6 | 19.0 | |
Off |
No photostability measurements available ... add one!
PAmKate was derived from mKate with the following mutations: F80W/S143N/S158G/*230LextN
amino acid numbers relative to eqFP578. show relative to mKate
To convert mKate into a PAFP, we mutated amino acid residues at positions 69, 84, 148, 165, 167, 181, and 203 (numbering follows the alignment with green fluorescent protein (GFP) , which were crucial for photoactivation of the chromophore in PAmCherry1.
Gunewardene et al. (2011)
(2011). Biophysical Journal, 101(6) , 1522-1528. doi: 10.1016/j.bpj.2011.07.049. Article Pubmed
(2018). Journal of the American Chemical Society, , . doi: 10.1021/jacs.8b05960. Article Pubmed
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