|Monomer||Entacmaea quadricolor||26.3 kDa||-|
|State||Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
No photostability measurements available ... add one!
PAmKate was derived from mKate with the following mutations: F80W/S143N/S158G/*230LextN
amino acid numbers relative to eqFP578. show relative to mKate
To convert mKate into a PAFP, we mutated amino acid residues at positions 69, 84, 148, 165, 167, 181, and 203 (numbering follows the alignment with green fluorescent protein (GFP) , which were crucial for photoactivation of the chromophore in PAmCherry1.
Gunewardene et al. (2011)
(2011). Biophysical Journal, 101(6) , 1522-1528. doi: 10.1016/j.bpj.2011.07.049. Article Pubmed
(2018). Journal of the American Chemical Society, , . doi: 10.1021/jacs.8b05960. Article Pubmed
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