Slow-FT is a timer fluorescent protein published in 2009, derived from Discosoma sp.. It is reported to be a monomer.
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Slow-FT was derived from
mCherry with the following mutations: M18V/E30V/K70R/L83W/A175V
amino acid numbers relative to
DsRed. show relative to mCherry
Medium-FT and Slow-FT exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 °C, the maxima of the blue fluorescence is observed at 9.8 h for slow-FT. The half-maxima of the red fluorescence is reached at 28 h.
Subach et al. (2009)
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Nature Chemical Biology, 5(2) , 118-126. doi: 10.1038/nchembio.138.
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