Monomeric fluorescent timers that change color from blue to red report on cellular trafficking

Subach Fv, Subach Om, Gundorov Is, Morozova Ks, Piatkevich Kd, Cuervo Am, Verkhusha Vv

(2009). Nature Chemical Biology, 5(2) , 118-126. doi: 10.1038/nchembio.138. Article   Pubmed

    Primary Proteins:
  1. Fast-FT
  2. Medium-FT
  3. Slow-FT
Add photostability measurements

Excerpts

Fast-FT, Medium-FT and Slow-FT exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 °C, the maxima of the blue fluorescence is observed at 9.8 h for slow-FT. The half-maxima of the red fluorescence is reached at 28 h.

Fast-FT, Medium-FT and Slow-FT exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 °C, the maxima of the blue fluorescence is observed at 0.25 h for fast-FT. The half-maxima of the red fluorescence is reached at 7.1 h.

Fast-FT, Medium-FT and Slow-FT exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 °C, the maxima of the blue fluorescence is observed at 1.2 h for fast-FT. The half-maxima of the red fluorescence is reached at 3.9 h.