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Medium-FT was derived from mCherry with the following mutations: M23L/N28D/T48S/K75R/L88W/M155I/Q193L/L204M/Y219C/R225HM18L/N23D/T43S/K70R/L83W/M150I/Q188L/L199M/Y214C/R220H
amino acid numbers relative to mCherryDsRed. show relative to DsRedshow relative to mCherry
Fast-FT, Medium-FT and Slow-FT exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 °C, the maxima of the blue fluorescence is observed at 1.2 h for fast-FT. The half-maxima of the red fluorescence is reached at 3.9 h.