Versatile phenotype-activated cell sorting

Lee J, Liu Z, Suzuki Ph, Ahrens Jf, Lai S, Lu X, Guan S, St-Pierre F

(2020). Science Advances, 6(43) , eabb7438. doi: 10.1126/sciadv.abb7438. Article   Pubmed

    Primary Proteins:
  1. mGold
    Secondary Proteins:
  1. mCitrine,
  2. mVenus,
  3. YPet

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mCitrine 32.7 20.0 mW/mm2 LED Widefield 24.0
mGold 29.8 62.5 mW/mm2 LED Widefield 24.0
mVenus 10.1 62.5 mW/mm2 LED Widefield 24.0
mGold 218.0 20.0 mW/mm2 LED Widefield 24.0
mGold 95.8 20.0 mW/mm2 LED Widefield 24.0
mVenus 50.4 20.0 mW/mm2 LED Widefield 24.0
mVenus 24.1 20.0 mW/mm2 LED Widefield 24.0
mCitrine 27.3 20.0 mW/mm2 LED Widefield 24.0
YPet 30.3 20.0 mW/mm2 LED Widefield 24.0
YPet 58.0 20.0 mW/mm2 LED Widefield 24.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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Excerpts are snippets from publications that capture key information about a protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).