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Comparison List

mIrisFP

similar: IrisFP

mIrisFP is a multi-photochromic green fluorescent protein published in 2010, derived from Lobophyllia hemprichii. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Lobophyllia hemprichii 25.7 kDa -

FPbase ID: KDHFJ

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Green 486 516 47,000 0.54 25.38 5.4 14.0  
Red 546 578 33,000 0.59 19.47 7.6    
Green (Off)              
Red (Off)              

Transitions

From To Switch λ
Green (Off) Green 386
Green Red 405
Red (Off) Red 446
Green Green (Off) 486
Red Red (Off) 546

Photostability

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mIrisFP Sequence

mIrisFP was derived from mEosFP with the following mutations: A69V/K145I/F173S/Y189A

MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEIMYVRDGVLTGDIHMALLLEGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Excerpts

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Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

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External Resources

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