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Comparison List

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iRFP670

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Feb 22, 2020 20:51:48. Or view the full change history

iRFP670 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2013, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization	Organism	Molecular Weight	Cofactor
Dimer	Rhodopseudomonas palustris CGA009	34.5 kDa	Biliverdin

FPbase ID: Q5DYR

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
643	670	114,000	0.11	12.54	4.0

Edit States/Attributes

Photostability

t_1/2 (s)	Power	Light	Mode	In Cell	Fusion	˚C	Reference
290.0	200.0 (W)	Arc-lamp	Widefield	HeLa			Shcherbakova et al. (2016)

A caution on interpretation of photostability measurements

Add photostability info

iRFP670 Sequence

BLAST

iRFP670 was derived from iRFP702 with the following mutations: V112I/K174M/I247CV112I/K174M/I247C

MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGAFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHRHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVMLYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRIVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEPRAVPMAQRVAAEMFADFLSLHFTAAHHQR

GenBank: AGN32863

Excerpts

No excerpts have been added for iRFP670
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).