Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer

Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A

(2004). Biochemical Journal, 381(1) , 307-312. doi: 10.1042/bj20040321. Article   Pubmed

    Primary Proteins:
  1. KO
  2. MiCy
  3. mKO
  4. mMiCy
Add photostability measurements

Excerpts

Due to the high value and pH-resistance of the QE, MiCy would probably be a more efficient and reliable donor for FRET than ECFP. In addition, fluorescence lifetime measurement revealed that MiCy fluorescence displayed as a single exponential decay with a time constant of 3.4 ns.

Since all of the non-bioluminescent Anthozoan GFP-like proteins characterized so far form obligate tetramers (Labas et al 2002), the dimer formation of MiCy may indicate structural divergence of the protein family.