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YFP3 is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2005, derived from Aequorea victoria. It has moderate acid sensitivity.
No photostability measurements available ...
YFP3 was derived from
EYFP-F46L with the following mutations: V1adel/I47L/Q80R/S208F/V224L/L231E/D234N
amino acid numbers relative to
avGFP. show relative to EYFP-F46L
YFP3 possessed six mutations that resulted in an RRC almost fivefold greater than that of the parental pair.
YFP3 also conferred a roughly threefold improvement in FRET relative to the fast-maturing Venus YFP.
Nguyen & Daugherty (2005)
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Nature Biotechnology, 23(3) , 355-360. doi: 10.1038/nbt1066.
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