Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Tetramer | Echinophyllia sp. SC22 | 25.4 kDa | - |
State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|---|
Off | ||||||||
On | 496 | 518 | 39,094 | 0.77 | 30.1 | 2.9 |
No photostability measurements available ... add one!
rsFastLime was derived from Dronpa with the following mutations: V157G
In the fluorescent equilibrium state, we find that the chromophore adopts exclusively the cis isomeric form. Surprisingly, the chromophore environment of Dronpa is almost identical to that of KikG, a fluorescent protein that does not exhibit photoswitching. Furthermore, we find structural similarities to asFP595 that point to a cis–trans isomerization of the chromophore as a key event in photochromic switching. Based on this assumption and the obtained structural information we generated rsFastLime (Dronpa-V157G) and Dronpa-M159T [aka Dronpa-2], two variants that exhibit strongly accelerated switching kinetics. Our findings support the view of a photoinduced cis–trans isomerization of the chromophore in Dronpa.
Stiel et al. (2007)
(2007). Biochemical Journal, 402(1) , 35-42. doi: 10.1042/bj20061401. Article Pubmed
(2008). Nature Biotechnology, 26(9) , 1035-1040. doi: 10.1038/nbt.1493. Article Pubmed
Something missing or incorrect? Submit a change Submit a change