|Tetramer||Echinophyllia sp. SC22||25.4 kDa||-|
|State||Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
No photostability measurements available ... add one!
rsFastLime was derived from Dronpa with the following mutations: V157G
In the fluorescent equilibrium state, we find that the chromophore adopts exclusively the cis isomeric form. Surprisingly, the chromophore environment of Dronpa is almost identical to that of KikG, a fluorescent protein that does not exhibit photoswitching. Furthermore, we find structural similarities to asFP595 that point to a cis–trans isomerization of the chromophore as a key event in photochromic switching. Based on this assumption and the obtained structural information we generated rsFastLime (Dronpa-V157G) and Dronpa-M159T [aka Dronpa-2], two variants that exhibit strongly accelerated switching kinetics. Our findings support the view of a photoinduced cis–trans isomerization of the chromophore in Dronpa.
Stiel et al. (2007)
(2007). Biochemical Journal, 402(1) , 35-42. doi: 10.1042/bj20061401. Article Pubmed
(2008). Nature Biotechnology, 26(9) , 1035-1040. doi: 10.1038/nbt.1493. Article Pubmed
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