Comparison List


rsFastLime is a photoswitchable green fluorescent protein published in 2007, derived from Echinophyllia sp. SC22.
Oligomerization Organism Molecular Weight Cofactor
Tetramer Echinophyllia sp. SC22 25.4 kDa -

FPbase ID: XJO9Z


State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
On 496 518 39,094 0.77 30.1     2.9


From To Switch λ
Off On 405
On Off 488


No photostability measurements available ... add one!

rsFastLime Sequence

rsFastLime was derived from Dronpa with the following mutations: V157G

GenBank: ABV80244
IPG: 13822637


In the fluorescent equilibrium state, we find that the chromophore adopts exclusively the cis isomeric form. Surprisingly, the chromophore environment of Dronpa is almost identical to that of KikG, a fluorescent protein that does not exhibit photoswitching. Furthermore, we find structural similarities to asFP595 that point to a cis–trans isomerization of the chromophore as a key event in photochromic switching. Based on this assumption and the obtained structural information we generated rsFastLime (Dronpa-V157G) and Dronpa-M159T [aka Dronpa-2], two variants that exhibit strongly accelerated switching kinetics. Our findings support the view of a photoinduced cis–trans isomerization of the chromophore in Dronpa.

Stiel et al. (2007)

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