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|Tetramer||Echinophyllia sp. SC22||25.5 kDa||-|
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PDM1-4 was derived from Dronpa with the following mutations: K145N
An attempt to find Dronpa variants with slower off-switching rate has led to the identification of PDM1-4. Unfortunately, PDM1-4 was prone to form tetramers and did not show improvement in photon output. In addition, PDM1-4 has worsened photostability and less contrast between on and off-states.
Chang et al. (2012)
(2010). Photochemical & Photobiological Sciences, 9(2) , 239. doi: 10.1039/b9pp00124g. Article Pubmed
(2012). Proceedings of the National Academy of Sciences, 109(12) , 4455-4460. doi: 10.1073/pnas.1113770109. Article Pubmed
(2012). Acta Crystallographica Section D Biological Crystallography, 68(12) , 1653-1659. doi: 10.1107/s0907444912039686. Article Pubmed
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