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| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Echinophyllia sp. SC22 | 25.5 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| On | 503 | 522 | 43,000 | 0.64 | 27.52 | |||
| Off |
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Padron was derived from rsFastLime with the following mutations: T59M/V60A/N94I/P141L/G155S/M159Y/F190S
To generate RSFPs with different switching properties, we used rsFastLime (Dronpa-V157G) as a template because of its favorable switching behavior, and its large dynamic range (67:1) in the fluorescence signal between the on- and the off-state.
Andresen et al. (2008)
At 37 °C, Padron* behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron*(star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron*(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron* is superior.
Andresen et al. (2008)
As its switching behavior is reverted to that of Dronpa, we termed this variant Padron*.
Andresen et al. (2008)
(2008). Nature Biotechnology, 26(9) , 1035-1040. doi: 10.1038/nbt.1493. Article Pubmed
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