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Comparison List

Padron

Padron is a photoswitchable green fluorescent protein published in 2008, derived from Echinophyllia sp. SC22.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Echinophyllia sp. SC22 25.5 kDa -

FPbase ID: 162KQ

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
On 503 522 43,000 0.64 27.52      
Off              

Transitions

From To Switch λ
Off On 405
On Off 488

Photostability

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Padron Sequence

Padron was derived from rsFastLime with the following mutations: T59M/V60A/N94I/P141L/G155S/M159Y/F190S

MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVKEGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICNATNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWELSTEKLYVRDGVLKSDGNYALSLEGGGHYRCDFKTTYKAKKVVQLPDYHSVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQAK

Excerpts

To generate RSFPs with different switching properties, we used rsFastLime (Dronpa-V157G) as a template because of its favorable switching behavior, and its large dynamic range (67:1) in the fluorescence signal between the on- and the off-state.

Andresen et al. (2008)

At 37 °C, Padron* behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron*(star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron*(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron* is superior.

Andresen et al. (2008)

As its switching behavior is reverted to that of Dronpa, we termed this variant Padron*.

Andresen et al. (2008)

Primary Reference

Additional References

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