Change History for Padron

• 2018-10-06 15:51, user: talley
  • added state transition Padron Off -503-> On
  • added state transition Padron On -405-> Off
• 2018-12-10 16:24, user: talley
  • changed protein Padron parent_organism_id: 46733->301887
  • changed protein Padron seq: H94I/S100I/R141L/N222Q
  • changed protein Padron switch_type: o->ps
  • added lineage Padron
• 2018-12-10 16:25, user: talley
  • added protein Padron references: Andresen et al. (2008)
  • added excerpt Andresen et al. (2008): To generate RSFPs with differe...
• 2018-12-10 16:26, user: talley
  • added excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ...
• 2018-12-10 16:27, user: talley
  • added excerpt Andresen et al. (2008): As its switching behavior is r...
• 2018-12-10 16:28, user: talley
  • changed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ... content: At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C)->At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained (Padron*) exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron* is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.
  • changed excerpt Andresen et al. (2008): As its switching behavior is r... content: As its switching behavior is reverted to that of Dronpa, we termed this variant Padron. ->As its switching behavior is reverted to that of Dronpa, we termed this variant Padron.
• 2018-12-10 16:32, user: talley
  • changed lineage Padron rght: 9->11
  • changed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ... content: At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained (Padron*) exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron* is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.->At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [Padron(star)] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.
• 2018-12-10 16:33, user: talley
  • changed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ... content: At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [Padron(star)] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.->At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron(star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.
• 2018-12-10 16:34, user: talley
  • changed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ... content: At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron(star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.->At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron (star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron (star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.
• 2018-12-10 16:37, user: talley
• 2018-12-10 16:38, user: talley
  • changed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ... content: At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron (star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron (star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.->At 37 °C, Padron behaves as a true monomer, although it has a slight tendency for dimerization at lower temperatures (∼15% dimer at 4 °C). The dimerization tendency was removed by exchanging hydrophobic amino acid residues that are located in potential dimer interfaces, namely Ile94, Ile100 and Leu141...The variant obtained [ Padron(star) ] exhibits a slightly reduced dynamic range in the switchable signal (off-state fluorescence is 2% instead of 0.7% of the on-state signal), and its bleaching per cycle amounts to 3% instead of 2%. Hence Padron(star) is particularly useful for applications at low temperatures; for most in vivo applications that require elevated temperatures, Padron is superior.
• 2023-11-07 16:03, user: Nuka.Kola
  • changed state transition Padron Off -503-> On trans_wave: 405->503
  • changed state transition Padron On -405-> Off trans_wave: 488->405
  • changed lineage Padron lft: 8->20
  • changed lineage Padron rght: 11->31
  • changed lineage Padron tree_id: 19->23
  • removed excerpt Andresen et al. (2008): To generate RSFPs with differe...
  • removed excerpt Andresen et al. (2008): At 37 °C, Padron behaves as a ...
  • removed excerpt Andresen et al. (2008): As its switching behavior is r...

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