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Comparison List

mTagBFP2

mTagBFP2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2011, derived from Entacmaea quadricolor. It has very low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.7 kDa -

FPbase ID: ZO7NN

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
399 454 50,600 0.64 32.38 2.7   2.6
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mTagBFP2 OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
49.8 ± 1.9 (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
53.0   Subach et al. (2011)
A caution on interpretation of photostability measurements
Add photostability info

mTagBFP2 Sequence

BLAST
MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN

Excerpts

No excerpts have been added for mTagBFP2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

Subach Om, Cranfill Pj, Davidson Mw, Verkhusha Vv

(2011). PLoS ONE, 6(12) , e28674. doi: 10.1371/journal.pone.0028674. Article   Pubmed

Additional References

  1. Quantitative assessment of fluorescent proteins

    Cranfill Pj, Sell Br, Baird Ma, Allen Jr, Lavagnino Z, De Gruiter Hm, Kremers G-J, Davidson Mw, Ustione A, Piston Dw

    (2016). Nature Methods, 13(7) , 557-562. doi: 10.1038/nmeth.3891. Article   Pubmed

External Resources

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