Comparison List


similar: SGFP2

msGFP2 is a basic (constitutively fluorescent) green fluorescent protein published in 2019, derived from Aequorea victoria.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.2 kDa -

FPbase ID: 4VSRL


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
489 509 52,200 0.59 30.8      


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msGFP2 Sequence



We previously generated msGFP, a monomeric superfolder derivative of EGFP. Unfortunately, compared to EGFP, msGFP and other superfolder GFP variants show faster photobleaching. We now describe msGFP2, which retains monomeric superfolder properties while being as photostable as EGFP. msGFP2 contains modified N- and C-terminal peptides that are expected to reduce nonspecific interactions. Compared to EGFP and mEGFP, msGFP2 is less prone to disturbing the functions of certain partner proteins.

Valbuena et al. (2019)

Primary Reference

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