| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | synthetic construct | 25.9 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 550 | 587 | 83,000 | 0.18 | 14.94 | 3.3 | 47.0 |
No photostability measurements available ... add one!
mScarlet3-S2 was derived from mScarlet3 with the following mutations: M67Q/M164H
amino acid numbers relative to mRed7. show relative to mScarlet3
mScarlet3-S2 achieved up to a 29-fold improvement in stability and is significantly more photostable than any other currently available RFP. Compared to the most photostable RFP, mScarlet-H1, 2, mScarlet3-S2 is up to 4.3-fold more photostable, has a 3.2-fold faster maturation, and exhibits slightly higher cellular brightness (1.1-fold).
Xu et al. (2024)
During the preparation of our manuscript, a preprint describing a photostability-enhanced single amino acid mutant of mScarlet3, named mYonghong, was submitted to Research Square. mYonghong corresponds to the mScarlet3-S0 variant (mScarlet3-M163H) in our manuscript. We present strong evidence that mScarlet3-S2 is up to 3.7 times more photostable than mScarlet3-S0 under illumination conditions for SIM microscopy.
Xu et al. (2024)
(2024). , , . doi: 10.21203/rs.3.rs-5629743/v1. Article
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