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mScarlet3

mScarlet3 is a basic (constitutively fluorescent) red fluorescent protein published in 2023, derived from synthetic construct. It is reported to be a rapidly-maturing monomer with low acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	synthetic construct	25.9 kDa	-

FPbase ID: 1AFJZ

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
569	592	104,000	0.75	78.0	4.5	37.0	4.0

Edit States/Attributes

Photostability

No photostability measurements available ... add one!

mScarlet3 Sequence

BLAST

mScarlet3 was derived from mScarlet-2A-84W with the following mutations: V2_G5delinsDST/K48R/T109A/K183R/Y194F/M227_K232delinsSGGSV2_G5delinsDST/K48R/T109A/K183R/Y194F/M227_K232delinsSGGS

MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGAVSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKMALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHSTGGSGGS

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for mScarlet3
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

mScarlet3: a brilliant and fast-maturing red fluorescent protein

Gadella Twj, Van Weeren L, Stouthamer J, Hink Ma, Wolters Ahg, Giepmans Bng, Aumonier S, Dupuy J, Royant A

(2023). Nature Methods, , . doi: 10.1038/s41592-023-01809-y. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for mScarlet3
Search RIKEN BRC for mScarlet3

Change history

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