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|Monomer||synthetic construct||25.8 kDa||-|
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To determine whether this improvement [replacing the N terminus with MDSTES and the C terminus with GSSGSS] extends to another FP, we examined mScarlet-I... The N-terminal peptide of mScarlet-I was replaced with MDSTEA because we found that MDSTES diminished the fluorescence (data not shown), and the C-terminal peptide was replaced with GGSGGS, yielding a variant that we termed mScarlet2-I. Although the difference between mScarlet-I and mScarlet-2I was less pronounced than the difference between mCherry and mCherry2C, the DH10B colonies expressing mSclarlet-2I were larger on average than those expressing mScarlet-I. The combined results indicate that the terminal peptides of EGFP can render some FPs cytotoxic.
Valbuena et al. (2019)
(2019). , , . doi: 10.1101/811588. Article
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