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mScarlet-I

mScarlet-I is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from synthetic construct. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer synthetic construct 26.4 kDa -

FPbase ID: 6VVTK

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
569 593 104,000 0.54 56.16 5.4 36.0 3.1
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mScarlet-I OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
76.0 (199 cells) 1.4 ± 0.2 (23 cells) U-2 OS Bindels et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
190.0 1.35 (W/cm2) Laser Spinning Disc Confocal H2A 37.0 Bindels et al. (2016)
225.0 6.9 (W/cm2) Arc-lamp Widefield H2A 37.0 Bindels et al. (2016)
A caution on interpretation of photostability measurements
Add photostability info

mScarlet-I Sequence

BLAST

mScarlet-I was derived from mScarlet with the following mutations: T74I

MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK
GenBank: APD76536
IPG: 129830965

Excerpts

The single amino acid substitution T74I found in mScarlet-I results in a marked maturation acceleration in cells, but at the cost of a moderate decrease in fluorescence quantum yield (0.54) and fluorescence lifetime (3.1 ns), although both values are still higher than those of all previously engineered bright mRFPs.

Bindels et al. (2016)

Primary Reference

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

Bindels Ds, Haarbosch L, Van Weeren L, Postma M, Wiese Ke, Mastop M, Aumonier S, Gotthard G, Royant A, Hink Ma, Gadella Twj

(2016). Nature Methods, 14(1) , 53-56. doi: 10.1038/nmeth.4074. Article   Pubmed

Additional References

  1. Spying on protein interactions in living cells with reconstituted scarlet light

    Wang S, Ding M, Xue B, Hou Y, Sun Y

    (2018). The Analyst, , . doi: 10.1039/c8an01223g. Article

  2. Optimal fluorescent protein tags for quantifying protein oligomerization in living cells

    Dunsing V, Luckner M, Zühlke B, Petazzi Ra, Herrmann A, Chiantia S

    (2018). Scientific Reports, 8(1) , 10634. doi: 10.1038/s41598-018-28858-0. Article   Pubmed

  3. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

    Mastop M, Bindels Ds, Shaner Nc, Postma M, Gadella Twj, Goedhart J

    (2017). Scientific Reports, 7(1) , 11999. doi: 10.1038/s41598-017-12212-x. Article   Pubmed

  4. Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells

    Chertkova Ao, Mastop M, Postma M, Van Bommel N, Van Der Niet S, Batenburg Kl, Joosen L, Gadella Twj, Okada Y, Goedhart J

    (2017). , , . doi: 10.1101/160374. Article

External Resources

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