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Comparison List

miRFP713

a.k.a. monomeric iRFP713

similar: iRFP713

miRFP713 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris CGA009. It has very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris CGA009 34.6 kDa Biliverdin

FPbase ID: 2EG15

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
690 713 99,000 0.07 6.93 3.5   0.89
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
980.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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miRFP713 Sequence

BLAST

miRFP713 was derived from iRFP713 with the following mutations: E301K/L302R/Q305E/V306R/W309T

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE
GenBank: MN701054

Excerpts

No excerpts have been added for miRFP713
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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