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Comparison List

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miRFP713

a.k.a. monomeric iRFP713

This protein has unmoderated changes.

miRFP713 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris CGA009. It has very low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Rhodopseudomonas palustris CGA009	34.6 kDa	Biliverdin

FPbase ID: 2EG15

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
690	713	99,000	0.07	6.93	3.5		0.89

Edit States/Attributes

Photostability

t_1/2 (s)	Power	Light	Mode	In Cell	Fusion	˚C	Reference
980.0	200.0 (W)	Arc-lamp	Widefield	HeLa		23.0	Matlashov et al. (2020)

A caution on interpretation of photostability measurements

Add photostability info

miRFP713 Sequence

BLAST

miRFP713 was derived from iRFP713 with the following mutations: E301K/L302R/Q305E/V306R/W309T E301K/L302R/Q305E/V306R/W309T

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE

GenBank: MN701054

Excerpts

No excerpts have been added for miRFP713
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for miRFP713
Search RIKEN BRC for miRFP713

Change history

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