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Comparison List

miRFP682

a.k.a. monomeric iRFP682

similar: iRFP682

miRFP682 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris CGA009 34.6 kDa Biliverdin

FPbase ID: X1DTP

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
663 682 91,000 0.112 10.19 4.5    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
500.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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miRFP682 Sequence

BLAST

miRFP682 was derived from iRFP682 with the following mutations: E301K/L302R/Q305E/V306R/W309T

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGVVIAEDRCAEVESKLGLHYPASAVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE

Excerpts

No excerpts have been added for miRFP682
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

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