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Comparison List

miRFP680

a.k.a. monomeric iRFP680

miRFP680 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2020, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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miRFP680 Spectrum Fluorescent protein miRFP680 excitation and emission spectra
Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris CGA009 34.6 kDa Biliverdin

FPbase ID: RAKQZ

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
661 680 94,000 0.145 13.63 4.5   1.67
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
980.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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miRFP680 Sequence

BLAST

miRFP680 was derived from iRFP713/V256C with the following mutations: E301K/L302R/Q305E/V306R/W309T

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE
GenBank: MN701052

Excerpts

No excerpts have been added for miRFP680
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Matlashov Me, Shcherbakova Dm, Alvelid J, Baloban M, Pennacchietti F, Shemetov Aa, Testa I, Verkhusha Vv

(2020). Nature Communications, 11(1) , 239. doi: 10.1038/s41467-019-13897-6. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

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