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Comparison List

iRFP713/V256C

iRFP713/V256C is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris CGA009 34.6 kDa Biliverdin

FPbase ID: 3G1CL

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
662 680 94,000 0.145 13.63 4.5    
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Photostability

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iRFP713/V256C Sequence

BLAST

iRFP713/V256C was derived from iRFP713 with the following mutations: V254C

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE

Excerpts

No excerpts have been added for iRFP713/V256C
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Allosteric effects of chromophore interaction with dimeric near-infrared fluorescent proteins engineered from bacterial phytochromes

Stepanenko Ov, Baloban M, Bublikov Gs, Shcherbakova Dm, Stepanenko Ov, Turoverov Kk, Kuznetsova Im, Verkhusha Vv

(2016). Scientific Reports, 6(1) , 18750. doi: 10.1038/srep18750. Article   Pubmed

Additional References

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External Resources

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