No spectrum has been submitted ... add a spectrum!
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Dendronephthya sp. | 25.6 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| Green | 488 | 505 | 21,000 | 0.7 | 14.7 | 6.6 | ||
| Red | 556 | 575 | 20,000 | 0.72 | 14.4 | 6.9 |
No photostability measurements available ... add one!
Dendra was derived from dendFP with the following mutations: L40A/L61V/Y95F/N121K/M123T/Y188A/S199G/G213A
To make dendGFP suitable for protein labeling we generated a monomeric variant, named Dendra (from Dendronephthya sp., red activatable), using mutagenesis strategy suggested for monomerization of green fluorescent protein Azami-Green. In contrast to a recently developed monomeric version of EosFP, called mEosFP, Dendra completely matures at 37 °C in both bacterial and mammalian cells. Bacterial colonies expressing Dendra can be irreversibly converted from green to red fluorescent states by irradiation with a 405-nm light using a fluorescent stereomicroscope. For purified Dendra, we achieved a 350-fold increase of red fluorescence and a fivefold decrease of green fluorescence resulting in a 1,400-fold contrast between the ground and activated states.
Gurskaya et al. (2006)
(2006). Nature Biotechnology, 24(4) , 461-465. doi: 10.1038/nbt1191. Article Pubmed
Something missing or incorrect? Submit a change Submit a change