Comparison List


a.k.a. LSSA12

LSSA12 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2022, derived from Aequorea victoria. It has low acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

FPbase ID: V81JD


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
398 511 38,700 0.38 14.71 4.6    


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LSSA12 Sequence

LSSA12 was derived from Hyperfolder YFP with the following mutations: G65S/Y203I/Q204E/E222D/R223F
amino acid numbers relative to avGFP. show relative to Hyperfolder YFP

GenBank: OP373688


LSSA12 was more stable in guanidinium HCl (GdnHCl) than mT-Sapphire, mAmetrine, and eGFP (Extended Data Fig. 6e). Site-directed mutagenesis confirmed the functional importance of the E222D and G65S mutations in LSSA12 (Extended Data Fig. 6f). LSSmGFP and LSSA12 enjoy similar advantages as hfYFP, including the absence of cysteine residues, low pKa, tolerance of fixatives, high chemical and thermal stability, and a single excitation band.

Campbell et al. (2022)

Primary Reference

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