Comparison List


a.k.a. LSSmGFP

LSSmGFP is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2022, derived from Aequorea victoria. It has low acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

FPbase ID: SGW8V


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
400 510 36,900 0.48 17.71 4.7    


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LSSmGFP Sequence

LSSmGFP was derived from Hyperfolder YFP with the following mutations: T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
amino acid numbers relative to avGFP. show relative to Hyperfolder YFP

GenBank: OP373689


Benefiting from the hfYFP crystal structure, we eliminated hfYFP’s 514-nm excitability and produced two exclusively 405-nm excitable GFPs with [a large Stokes shift], LSSA12 and LSSmGFP. These LSS FPs overcome the cross-excitation problem of mT-Sapphire (Fig. 5c–e) without sacrificing molecular brightness (Table 1). LSSmGFP has high chemical and thermodynamic stability and the same molecular brightness as mAmetrine, while lasting twice as long under laser-scanning confocal illumination before photobleaching (Extended Data Fig. 7g).

Campbell et al. (2022)

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