a.k.a. LSSmGFP
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Aequorea victoria | 26.9 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 400 | 510 | 36,900 | 0.48 | 17.71 | 4.7 |
No photostability measurements available ... add one!
LSSmGFP was derived from Hyperfolder YFP with the following mutations: T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
amino acid numbers relative to avGFP. show relative to Hyperfolder YFP
Benefiting from the hfYFP crystal structure, we eliminated hfYFP’s 514-nm excitability and produced two exclusively 405-nm excitable GFPs with [a large Stokes shift], LSSA12 and LSSmGFP. These LSS FPs overcome the cross-excitation problem of mT-Sapphire (Fig. 5c–e) without sacrificing molecular brightness (Table 1). LSSmGFP has high chemical and thermodynamic stability and the same molecular brightness as mAmetrine, while lasting twice as long under laser-scanning confocal illumination before photobleaching (Extended Data Fig. 7g).
Campbell et al. (2022)
(2022). Nature Methods, , . doi: 10.1038/s41592-022-01660-7. Article Pubmed
Something missing or incorrect? Submit a change Submit a change