| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Weak dimer | Aequorea victoria | 26.9 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 435 | 477 | 35,000 | 0.51 | 17.85 | 5.02 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 94.0 ± 2.4 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
No photostability measurements available ... add one!
CyPet was derived from ECFP with the following mutations: T9G/V11I/D19E/A87V/I167A/E172T/L194I/L231H
amino acid numbers relative to avGFP. show relative to ECFP
Nguyen and Daugherty (2005) reported the development of an improved FRET pair, YPet & CyPet, which had a sevenfold enhancement of the FRET signal. However, we discovered that most of the signal enhancement was due to enhanced dimerization of YPet to CyPet within the tethered construct, and was substantially reduced when we incorporated the monomerizing mutation previously developed to prevent the weak dimerization of GFP (Zacharias et al. 2002).
Ohashi et al. (2007)
(2005). Nature Biotechnology, 23(3) , 355-360. doi: 10.1038/nbt1066. Article Pubmed
(2007). Protein Science, 16(7) , 1429-1438. doi: 10.1110/ps.072845607. Article Pubmed
(2007). ChemBioChem, 8(10) , 1119-1121. doi: 10.1002/cbic.200700109. Article Pubmed
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