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mAzamiGreen

a.k.a. mAG

mAzamiGreen is a basic (constitutively fluorescent) green fluorescent protein published in 2003, derived from Galaxea fascicularis. It has high acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Galaxea fascicularis	25.9 kDa	-

FPbase ID: QRBKZ

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
492	505	41,800	0.81	33.86	6.2

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mAzamiGreen OSER Measurements

% Normal Cells	OSER/NE ratio	Cell Type	Reference
90.3 ± 4.7 (10000 cells)	-	HeLa	Cranfill et al. (2016)

Photostability

No photostability measurements available ... add one!

mAzamiGreen Sequence

BLAST

MSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVTEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTATSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVRDGVLKGDVNMALLLEGGGHYRCDFKTTYKAKKDVRLPDAHKVDHRIEILKHDKDYNKVKLYENAVARYSMLPSQAK

GenBank: BAD52002

UniProtKB: Q60I24

IPG: 3705442

Structure

PDB ID

Deposited: ,
Chromophore (QYG):

Excerpts

No excerpts have been added for mAzamiGreen
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

A Green-emitting Fluorescent Protein fromGalaxeidaeCoral and Its Monomeric Version for Use in Fluorescent Labeling

Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A

(2003). Journal of Biological Chemistry, 278(36) , 34167-34171. doi: 10.1074/jbc.m304063200. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for mAzamiGreen
Search RIKEN BRC for mAzamiGreen

Change history

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