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PAmKate

PAmKate is a photoactivatable red fluorescent protein published in 2011, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Entacmaea quadricolor	26.3 kDa	-

FPbase ID: DTAXR

Attributes

State	Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
On	586	628	25,000	0.18	4.5	5.6	19.0
Off

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Transitions

From	To	Switch λ
Off	On	405

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Photostability

No photostability measurements available ... add one!

PAmKate Sequence

BLAST

PAmKate was derived from mKate with the following mutations: F80W/S143N/S158G/*232LextN F80W/S143N/S158G/*230LextN
amino acid numbers relative to mKate eqFP578. show relative to eqFP578 show relative to mKate

MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRGDMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHKLN

Excerpts

To convert mKate into a PAFP, we mutated amino acid residues at positions 69, 84, 148, 165, 167, 181, and 203 (numbering follows the alignment with green fluorescent protein (GFP) , which were crucial for photoactivation of the chromophore in PAmCherry1.

Gunewardene et al. (2011)