Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | Aequorea victoria | 26.8 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
406 | 526 | 45,000 | 0.58 | 26.1 | 6.0 | 1.8 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
90.0 ± 4.5 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
2.8 | Ai et al. (2008) |
mAmetrine was derived from EGFP with the following mutations: S30R/Y39N/T50S/T65S/S72A/F99S/N105S/K107R/Y145M/S147V/H148W/N149D/V150A/M153T/V163A/R168E/I171V/S175G/H181A/P192S/T203F/A206K/S208F/K214Q/L231P
amino acid numbers relative to avGFP. show relative to EGFP
During the development of the violet-excitable blue-fluorescing A. victoria GFP variant mKalama1, we fortuitously discovered a long-Stokes-shift violet-excitable yellow-fluorescing variant. We subjected this new fluorescent protein to aggressive directed protein evolution and eventually produced a brightly fluorescent variant with 20 mutations relative to GFP. The final variant, designated as mAmetrine, retained the property of violet excitation owing to a protonated ground state, and yellow emission resulting from excited state proton transfer and π-π stacking interactions with the chromophore.
Ai et al. (2008)
(2008). Nature Methods, 5(5) , 401-403. doi: 10.1038/nmeth.1207. Article
(2018). The Analyst, , . doi: 10.1039/c8an01223g. Article
(2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article Pubmed
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