(2008). Nature Methods, 5(5) , 401-403. doi: 10.1038/nmeth.1207. Article
|Protein (state)||t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C|
During the development of the violet-excitable blue-fluorescing A. victoria GFP variant mKalama1, we fortuitously discovered a long-Stokes-shift violet-excitable yellow-fluorescing variant. We subjected this new fluorescent protein to aggressive directed protein evolution and eventually produced a brightly fluorescent variant with 20 mutations relative to GFP. The final variant, designated as mAmetrine, retained the property of violet excitation owing to a protonated ground state, and yellow emission resulting from excited state proton transfer and π-π stacking interactions with the chromophore.