a.k.a. FOLD6
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Weak dimer | Aequorea victoria | 26.8 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 505 | 515 | 118,200 | 0.73 | 86.29 | 5.9 | 25.0 |
No photostability measurements available ... add one!
FOLD6 was derived from mGreenLantern with the following mutations: C48S/V68L/C70V/K101E/T105Y/S147P/K149N/K206V/K221L/R223F/D234N
amino acid numbers relative to avGFP. show relative to mGreenLantern
In contrast to Clover (Extended Data Fig. 5a), sfGFP (Extended Data Fig. 5b), and all other avFPs we had tested, FOLD6 required 10 min to [denature] in 1 M NaOH solution (pH ≥ 13) (Extended Data Fig. 5c). We performed time-course experiments with eight different FPs and found that Clover, eYFP, and even mGL and hfYFP, denatured right away in 1 M NaOH (Extended Data Fig. 5d-g).
Campbell et al. (2022)
(2022). Nature Methods, , . doi: 10.1038/s41592-022-01660-7. Article Pubmed
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