Comparison List


a.k.a. FOLD6

FOLD6 is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2022, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Weak dimer Aequorea victoria 26.8 kDa -

FPbase ID: C5EUY


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
505 515 118,200 0.73 86.29 5.9 25.0  


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FOLD6 Sequence

FOLD6 was derived from mGreenLantern with the following mutations: C48S/V68L/C70V/K101E/T105Y/S147P/K149N/K206V/K221L/R223F/D234N
amino acid numbers relative to avGFP. show relative to mGreenLantern



Deposited: ,


In contrast to Clover (Extended Data Fig. 5a), sfGFP (Extended Data Fig. 5b), and all other avFPs we had tested, FOLD6 required 10 min to [denature] in 1 M NaOH solution (pH ≥ 13) (Extended Data Fig. 5c). We performed time-course experiments with eight different FPs and found that Clover, eYFP, and even mGL and hfYFP, denatured right away in 1 M NaOH (Extended Data Fig. 5d-g).

Campbell et al. (2022)

Primary Reference

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