|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|56.5 (69 cells)||-||HeLa||Costantini et al. (2015)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|34.0||Subach et al. (2008)|
TagBFP was derived from TagRFP with the following mutations: N20D/M63L/R67K/P127T/N143F/M146T/S158N/G168S/C172A/F174I/H197Y/D206N/K207N/*230LextN
amino acid numbers relative to eqFP578. show relative to TagRFP
Note: Subach et al (2008), derives mTagBFP from TagRFP, but Fig 1 depicts TagRFP (and therefore mTagBFP) with two glutamic acids in the N terminus instead of one, as shown in the original TagRFP paper (Merzlyak et al 2007). At Snapgene and in the vast majority of variants seen on Addgene, there is only a single glutamic acid. Here, we use a single glutamic acid in the N terminus, in contrast to the original paper.
No excerpts have been added for TagBFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).