Designed active-site library reveals thousands of functional GFP variants

Weinstein Jy, Gomez Aldaravi Cm, Lipsh-Sokolik R, Hoch Sy, Liebermann D, Nevo R, Weissman H, Petrovich-Kopitman E, Margulies D, Ivankov D, Mccandlish D, Fleishman Sj

(2022). , , . doi: 10.1101/2022.10.11.511732. Article

    Primary Proteins:
  1. htFuncLib_acid.1
  2. htFuncLib_acid.2
  3. htFuncLib_acid.3
  4. htFuncLib_acid.4
  5. htFuncLib_acid.5
  6. htFuncLib_acid.6
  7. htFuncLib_basic.1
  8. htFuncLib_basic.2
  9. htFuncLib_basic.3
  10. htFuncLib_basic.4
  11. htFuncLib_basic.6
  12. htFuncLib_bleach.1
  13. htFuncLib_bleach.2
  14. htFuncLib_bleach.3
  15. htFuncLib_bleach.4
  16. htFuncLib_bleach.5
  17. htFuncLib_bleach.6
  18. htFuncLib_em-blue-shift.1
  19. htFuncLib_em-blue-shift.2
  20. htFuncLib_fast.1
  21. htFuncLib_fast.2
  22. htFuncLib_fast.3
  23. htFuncLib_fast.4
  24. htFuncLib_mid.10
  25. htFuncLib_mid.11
  26. htFuncLib_mid.12
  27. htFuncLib_mid.13
  28. htFuncLib_mid.2
  29. htFuncLib_mid.3
  30. htFuncLib_mid.4
  31. htFuncLib_mid.5
  32. htFuncLib_mid.6
  33. htFuncLib_mid.7
  34. htFuncLib_mid.8
  35. htFuncLib_mid.9
  36. htFuncLib_photostable.1
  37. htFuncLib_photostable.2
  38. htFuncLib_sf:acid.3
  39. htFuncLib_sf:acid.4
  40. htFuncLib_sf:acid.5
  41. htFuncLib_sf:bleach.1
  42. htFuncLib_sf:bleach.2
  43. htFuncLib_sf:bleach.3
  44. htFuncLib_sf:em-blue-shift.1
  45. htFuncLib_sf:em-blue-shift.2
  46. htFuncLib_sf:fast.3
  47. htFuncLib_sf:mid.10
  48. htFuncLib_sf:mid.11
  49. htFuncLib_sf:mid.12
  50. htFuncLib_sf:mid.5
  51. htFuncLib_sf:mid.7
  52. htFuncLib_sf:mid.9
  53. htFuncLib_sf:photostable.1
  54. htFuncLib_sf:photostable.2
  55. htFuncLib_sf:thermo.2
  56. htFuncLib_sf:thermo.9
  57. htFuncLib_slow.1
  58. htFuncLib_slow.2
  59. htFuncLib_slow.3
  60. htFuncLib_slow.4
  61. htFuncLib_slow.5
  62. htFuncLib_thermo.1
  63. htFuncLib_thermo.2
  64. htFuncLib_thermo.3
  65. htFuncLib_thermo.4
  66. htFuncLib_thermo.5
  67. htFuncLib_thermo.6
  68. htFuncLib_thermo.8
  69. htFuncLib_thermo.9
  70. PROSS-eGFP

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
htFuncLib_fast.2 18.1 22.7 mW LED Widefield
htFuncLib_slow.3 18.6 22.7 mW LED Widefield
htFuncLib_fast.3 39.6 22.7 mW LED Widefield
htFuncLib_bleach.2 15.5 22.7 mW LED Widefield
htFuncLib_bleach.6 20.7 22.7 mW LED Widefield
htFuncLib_mid.2 27.7 22.7 mW LED Widefield
htFuncLib_basic.3 47.2 22.7 mW LED Widefield
htFuncLib_thermo.9 44.4 22.7 mW LED Widefield
htFuncLib_bleach.4 18.3 22.7 mW LED Widefield
htFuncLib_mid.3 28.0 22.7 mW LED Widefield
htFuncLib_photostable.2 67.0 22.7 mW LED Widefield
htFuncLib_bleach.1 11.4 22.7 mW LED Widefield
htFuncLib_fast.4 6.9 22.7 mW LED Widefield
htFuncLib_thermo.5 19.9 22.7 mW LED Widefield
htFuncLib_basic.6 20.8 22.7 mW LED Widefield
htFuncLib_acid.1 23.4 22.7 mW LED Widefield
htFuncLib_thermo.4 30.9 22.7 mW LED Widefield
htFuncLib_acid.2 21.3 22.7 mW LED Widefield
htFuncLib_thermo.6 20.5 22.7 mW LED Widefield
htFuncLib_bleach.5 19.6 22.7 mW LED Widefield
htFuncLib_slow.5 14.5 22.7 mW LED Widefield
htFuncLib_thermo.8 19.6 22.7 mW LED Widefield
htFuncLib_slow.4 28.5 22.7 mW LED Widefield
htFuncLib_slow.2 31.0 22.7 mW LED Widefield
htFuncLib_mid.4 25.7 22.7 mW LED Widefield
htFuncLib_mid.5 25.2 22.7 mW LED Widefield
htFuncLib_acid.3 20.9 22.7 mW LED Widefield
htFuncLib_acid.4 19.7 22.7 mW LED Widefield
htFuncLib_mid.6 21.3 22.7 mW LED Widefield
htFuncLib_mid.7 32.1 22.7 mW LED Widefield
htFuncLib_basic.4 21.8 22.7 mW LED Widefield
htFuncLib_em-blue-shift.2 23.5 22.7 mW LED Widefield
htFuncLib_mid.8 33.4 22.7 mW LED Widefield
htFuncLib_acid.5 38.5 22.7 mW LED Widefield
htFuncLib_em-blue-shift.1 22.7 22.7 mW LED Widefield
htFuncLib_mid.9 43.3 22.7 mW LED Widefield
htFuncLib_mid.10 35.8 22.7 mW LED Widefield
htFuncLib_mid.11 53.6 22.7 mW LED Widefield
htFuncLib_thermo.2 60.0 22.7 mW LED Widefield
htFuncLib_slow.1 18.2 22.7 mW LED Widefield
htFuncLib_thermo.1 43.7 22.7 mW LED Widefield
htFuncLib_thermo.3 31.2 22.7 mW LED Widefield
htFuncLib_basic.2 23.7 22.7 mW LED Widefield
htFuncLib_acid.6 37.3 22.7 mW LED Widefield
htFuncLib_mid.12 29.7 22.7 mW LED Widefield
htFuncLib_basic.1 34.3 22.7 mW LED Widefield
htFuncLib_bleach.3 17.9 22.7 mW LED Widefield
htFuncLib_mid.13 34.0 22.7 mW LED Widefield
htFuncLib_photostable.1 75.1 22.7 mW LED Widefield
htFuncLib_fast.1 27.6 22.7 mW LED Widefield
PROSS-eGFP 37.2 22.7 mW LED Widefield
htFuncLib_sf:acid.4 23.8 22.7 mW LED Widefield
htFuncLib_sf:acid.5 23.9 22.7 mW LED Widefield
htFuncLib_sf:mid.9 31.6 22.7 mW LED Widefield
htFuncLib_sf:em-blue-shift.2 30.9 22.7 mW LED Widefield
htFuncLib_sf:photostable.1 39.1 22.7 mW LED Widefield
htFuncLib_sf:mid.11 36.4 22.7 mW LED Widefield
htFuncLib_sf:bleach.2 37.7 22.7 mW LED Widefield
htFuncLib_sf:fast.3 34.6 22.7 mW LED Widefield
htFuncLib_sf:thermo.2 47.0 22.7 mW LED Widefield
htFuncLib_sf:bleach.3 34.7 22.7 mW LED Widefield
htFuncLib_sf:acid.3 28.0 22.7 mW LED Widefield
htFuncLib_sf:photostable.2 36.7 22.7 mW LED Widefield
htFuncLib_sf:em-blue-shift.1 11.8 22.7 mW LED Widefield
htFuncLib_sf:bleach.1 26.7 22.7 mW LED Widefield
htFuncLib_sf:mid.12 40.1 22.7 mW LED Widefield
htFuncLib_sf:mid.5 26.6 22.7 mW LED Widefield
htFuncLib_sf:thermo.9 25.3 22.7 mW LED Widefield
htFuncLib_sf:mid.7 36.5 22.7 mW LED Widefield
htFuncLib_sf:mid.10 30.4 22.7 mW LED Widefield
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

No excerpts have been added from this reference.
Excerpts are snippets from publications that capture key information about a protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).