Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity

Gorbachev Da, Staroverov Db, Lukyanov Ka, Sarkisyan Ks

(2020). International Journal of Molecular Sciences, 21(22) , 8800. doi: 10.3390/ijms21228800. Article   Pubmed

    Primary Proteins:
  1. SuperNova2
Add photostability measurements


A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.