|Monomer||Anthomedusae sp. DC-2005||26.6 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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SuperNova2 was derived from SuperNova Red with the following mutations: M1_E2insGS/S10R
A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.
Gorbachev et al. (2020)
(2020). International Journal of Molecular Sciences, 21(22) , 8800. doi: 10.3390/ijms21228800. Article Pubmed
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