Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

Oliinyk Os, Shemetov Aa, Pletnev S, Shcherbakova Dm, Verkhusha Vv

(2019). Nature Communications, 10(1) , 279. doi: 10.1038/s41467-018-08050-8. Article   Pubmed

    Primary Proteins:
  1. miRFP670nano
Add photostability measurements

Excerpts

Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag.

miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue–green optogenetic tools