|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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miRFP670nano was derived from NpR3784g with the following mutations: V7M/F25C/M26V/Y27F/P31E/S41A/A48S/N51K/Q55R/T57R/I72Y/G82N/H87Y/N99I/N117H/L136Q/Q139V/Q148_L149del
Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag.
miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue–green optogenetic tools