The 2018 correlative microscopy techniques roadmap

Ando T, Bhamidimarri Sp, Brending N, Colin-York H, Collinson L, De Jonge N, De Pablo Pj, Debroye E, Eggeling C, Franck C, Fritzsche M, Gerritsen H, Giepmans Bng, Grunewald K, Hofkens J, Hoogenboom Jp, Janssen Kpf, Kaufman R, Klumpermann J, Kurniawan N, Kusch J, Liv N, Parekh V, Peckys Db, Rehfeldt F, Reutens Dc, Roeffaers Mbj, Salditt T, Schaap Iat, Schwarz Us, Verkade P, Vogel Mw, Wagner R, Winterhalter M, Yuan H, Zifarelli G

(2018). Journal of Physics D: Applied Physics, 51(44) , 443001. doi: 10.1088/1361-6463/aad055. Article

    Secondary Proteins:
  1. mEos4a,
  2. mEos4b
Add photostability measurements

Excerpts

Osmium-resistant probes for fluorescence and electron microscopy—the fluorescent EosFP derivative mEos4 has recently been described to withstand osmium treatment during EM processing. The mEos4 molecule is therefore still fluorescent in a well-stained resin-embedded sample. In addition, it is compatible with super resolution photoactivated localization microscopy.

Osmium-resistant probes for fluorescence and electron microscopy—the fluorescent EosFP derivative mEos4 has recently been described to withstand osmium treatment during EM processing. The mEos4 molecule is therefore still fluorescent in a well-stained resin-embedded sample. In addition, it is compatible with super resolution photoactivated localization microscopy.